AP Biology Investigation #9: Restriction Enzyme Analysis

In stock
code
118-0189

In this lab students will understand the action and specificity of restriction enzymes, while learning the components involved in the process of DNA electrophoresis and the role of each component as it applies to the entire process of electrophoresis. Students will perform the electrophoresis process on DNA samples treated with different restriction enzymes and construct a standard curve using a known DNA sample while determining the approximate size of the DNA fragments in unknown samples. Meets AP Science Practices 3 and 6, and Big Idea 3. Materials provided for eight lab groups.

Kit Includes:

200mL Prepared Agarose, 0.8%
500mL TBE buffer, 5X
60mL DNA Stain, 20X
DNA Samples, 100ul each:
DNA Marker (Lambda DNA HindIII digest)
Sample #1 (Lambda DNA)
Sample #2 (Lambda DNA BstEII digest)
Sample #3 (Lambda DNA EcoRI digest)
Sample #4 (Lambda DNA BstEII digest)

Materials Needed but not Supplied:

Agarose electrophoresis chamber Microwave or hot water bath
Electrophoresis power supply Distilled or deionized water
Micropipettes w/micropipette tips, 10ul
Gel staining trays or similar (large enough container to accommodate flat agarose gels)

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Product Questions

No questions yet. Be the first to ask the question!

How long will my delivery take?

Every order received by Westlab before 12pm PST will be dispatched on the next business day.

 

Shipping Time Based on Delivery Location

British Columbia/local shipments deliver in 1-2 business days

Alberta shipments deliver in 2-3 business days

Prairies (SK & MB) shipments deliver in 3-4 business days

Ontario/Quebec shipments deliver in 4-5 business days

Maritimes (NL, PEI, NS & NB) shipments deliver in 5-7 business days

Territories (YT, NT & NU) shipments depend on shipping service & location

*Ask our team for more information about shipping up north

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