DNA fingerprinting is one of the strongest forms of evidence in forensic investigations. In this activity students will perform agarose gel electrophoresis on four DNA samples to determine if DNA from suspects matches that of DNA found at a crime scene. Students will also learn about restriction enzymes and the importance of these enzymes in the DNA fingerprinting process.
200mL Prepared Agarose, 0.8% Solution
500mL Tris-Borate-EDTA Buffer, 5X
60mL DNA Stain, 20X
10 μg Crime Scene DNA
10 μg Victim DNA
10 μg Suspect #1 DNA
10 μg Suspect #2 DNA